Polarized light is known to be a contrast enhancing technique which helps in improves the quality of the image. Also, the dark field andbright field illumination, differential interference contrast and phase contrast.
Polarizing light microscopy are having a high degree of sensitivity and also can be utilized for both quantitative and qualitative microscopes. The qualitative scope is very popular in practice. This scope is primarily used in crystallography. Also, they are used in geologists, mineralogists and chemists. It is also best known for being used in composites, ceramics, mineral fibers, polymers, wood, urea and a group f other macromolecules and structural assemblies.
Polarized light scope basically enhances the image contrast, betters the image quality. It has a nice working.
Working of Polarized Microscopy:
A polarizer comes in between the light source and test specimen. Then, it is converted into plane polarized light before it collides with the sample. This light then falls on a doubly refracting specimen which then generates a two wave components. They are at right angles with each other. The two waves with which it is at right angles are known as ordinary and extraordinary light rays. The waves pass through the sample in different phases.
Then, they are then combined using a constructive and a destructive interference by the use of an analyzer. This whole phenomenon will lead to one final generation of a very high contrast image.